Journal: Frontiers in Pharmacology

Article Title: Role of albumin in regulating platelet function

doi: 10.3389/fphar.2026.1734694

Figure Lengend Snippet: Hypoproteinemia was found to promote platelet activation. (A–E) Wash platelet aggregation in WT, ADR and ADR Albumin mice induced by thrombin (A) , U46619 (B) , PAR4-AP (C) , collagen (D) and ADP (E) (n = 5 independent experimental animals). (F–H) Thrombin (F) , U46619 (G) and PAR4-AP (H) induced integrin αIIbβ3 activation in washed platelets from mice (n = 5 independent experimental animals). (I–K) Thrombin (I) , U46619 (J) and PAR4-AP (K) induced P-selectin release from washed platelets from mice (n = 5 independent experimental animals). (L–O) ATP release from washed platelets in mice was induced by thrombin (L) , U46619 (M) , PAR4-AP (N) and collagen (O) (n = 5 independent experimental animals). (P–R) Wash platelet spreading in mice induced by U46619 (P) , mean spreading area of individual platelets (Q) , and proportion of spreading platelets to total platelets in the field of view (R) (n = 3 independent experimental animals). Differences between groups were assessed by one-way ANOVA followed by Dunnett’s post hoc test. Statistics are presented as mean ± SEM. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Rabbit antibodies against phospho-PKC substrate (2261), phospho-Akt (Ser473) (11E7), Akt (pan) (11E7), PKC alpha (2056), GAPDH (5174), cell lysate buffer (9803), and PAR4-AP (2328) were sourced from Cell Signaling Technology, Inc. (Beverly, MA, United States).

Techniques: Activation Assay

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 4. Screening of key prognostic genes. (A) Protein–protein interaction (PPI) analysis. (B) KEGG analysis. (C) Expression of F2RL3 between tumor and normal tissues. (D) Expression of F2RL3 in GC stages. (E) Kaplan-Meier (K-M) analysis of F2RL3.

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Expressing

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 5. F2RL3 expression level in human GC tissue. (A) F2RL3 expression detected by Western blotting (n = 6). (B) Bar chart

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Expressing, Western Blot

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 6. F2RL3 expression level in human GC cells. (A) F2RL3 expression detected by Western blotting. (B) Bar chart showing F2RL3

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Expressing, Western Blot

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 7. Effects of F2RL3 knockdown on the EMT of GC cells. (A) F2RL3 expression detected by Western blotting. (B) Bar chart

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Knockdown, Expressing, Western Blot

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 8. Effects of F2RL3 expression knockdown on angiogenesis. (A) The activity of Human Umbilical Vein Endothelial Cells

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Expressing, Knockdown, Activity Assay

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 9. F2RL3 regulates the expression of Rap1/MAPK signaling pathway in GC cells. (A) Expression of proteins related to F2RL3

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Expressing

Journal: Frontiers in bioscience (Landmark edition)

Article Title: F2RL3 Regulates Epithelial-Mesenchymal Transition and Angiogenesis in Gastric Cancer through the Rap1/MAPK Signaling Pathway.

doi: 10.31083/j.fbl2905177

Figure Lengend Snippet: Fig. 10. Effects of the Rap1/MAPK signaling pathway on the EMT of GC cells. (A) Expression of F2RL3, E-cadherin, vimentin, and Snail detected by Western blotting. (B) Bar chart showing the expression of F2RL3, vimentin, E-cadherin, and Snail. (C) Activity

Article Snippet: Then the sections were incubated with F2RL3 primary antibody (#25306-1-AP; Proteintech, Rosemont, IL, USA), followed by incubation with secondary antibody.

Techniques: Expressing, Western Blot, Activity Assay